منابع مشابه
Transformation of Saccharomyces cerevisiae by electroporation.
A method for introducing heterologous DNA into Saccharomyces cerevisiae rapidly and efficiently by electroporation was developed. Transformant colonies appeared somewhat sooner than by the LiCl or spheroplast transformation method, and the time spent in manipulation was much less than for these two methods. The pores in the cell membrane formed by the high voltage of electroporation were reseal...
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A key mission of computer science is to enable people realize their creative ideas as naturally and painlessly as possible. Software engineering is at the center of this mission — software technologies enable reification of ideas into working systems. As computers become ubiquitous, both in availability and the aspects of human lives they touch, the quantity and diversity of ideas also rapidly ...
متن کاملGenetic transformation of colony type in Xanthomonas phaseoli.
The first demonstration of transformation (Griffith, 1928) was concerned with type-specific capsular polysaccharides of pneumococci. Such transformations of capsular types usually are accompanied by a change in colony type since most investigators have studied transformation of R cells which produce little or no capsular material. Coiacomitant transformation of capsular and colony type has been...
متن کاملHigh-throughput transformation of Saccharomyces cerevisiae using liquid handling robots
Saccharomyces cerevisiae (budding yeast) is a powerful eukaryotic model organism ideally suited to high-throughput genetic analyses, which time and again has yielded insights that further our understanding of cell biology processes conserved in humans. Lithium Acetate (LiAc) transformation of yeast with DNA for the purposes of exogenous protein expression (e.g., plasmids) or genome mutation (e....
متن کاملRapid single-colony whole-genome sequencing of bacterial pathogens
OBJECTIVES As a result of the introduction of rapid benchtop sequencers, the time required to subculture a bacterial pathogen to extract sufficient DNA for library preparation can now exceed the time to sequence said DNA. We have eliminated this rate-limiting step by developing a protocol to generate DNA libraries for whole-genome sequencing directly from single bacterial colonies grown on prim...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1991
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/19.8.1945